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Image Search Results
Journal: Journal of nanobiotechnology
Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration.
doi: 10.1186/s12951-023-02125-5
Figure Lengend Snippet: Fig. 1 Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs
Article Snippet: Briefly, 107 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and
Techniques: Light Microscopy, Flow Cytometry, Staining
Journal: bioRxiv
Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells
doi: 10.64898/2026.06.25.733625
Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.
Article Snippet: Antibodies against the following human antigens were used:
Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay
Journal: Journal of cellular and molecular medicine
Article Title: Insights From Amniotic and Umbilical Cord Mesenchymal Stem Cells in Wound Healing.
doi: 10.1111/jcmm.70679
Figure Lengend Snippet: FIGURE 1 | Isolation and characterisation of UC-MSCs and A-MSCs. (A) Both UC-MSCs and A-MSCs exhibit a typical spindle-shaped morphol- ogy. Scale bar = 100 μm. (B) The proliferation of the two cells was evaluated by CCK8 assay. (C) These cells are positively stained for the common MSC-associated markers, CD73, CD90, CD105, and have no expression of haematopoietic markers CD45, CD34 and HLA-DR. (D) Following the cul- ture in a specific differentiation medium for 2–3 weeks, the isolated UC-MSCs and A-MSCs successfully differentiated into mesenchymal derivatives, including osteoblasts (Alizarin Red labeling), adipocytes (Oil Red O labeling) and chondrocytes (Alcian blue). Scale bar = 50 μm. (E) The transwell was adopted to evaluate the migration ability of the two types of cells. Scale bar = 50 μm. (F) The wound healing assays were further adopted for the migration ability of the cells. Scale bar = 100 μm.
Article Snippet: Additionally, phenotypic characteristics of UC- MSCs and A- MSCs at passage 3 were analysed by flow cytometry using antibodies against CD14 (BD Biosciences; 555397), CD34 (BD Biosciences; 555822), CD73 (BioLegend; 344004),
Techniques: Isolation, CCK-8 Assay, Staining, Expressing, Labeling, Migration