Journal: Oncotarget

Article Title: New strategy to rescue the inhibition of osteogenesis of human bone marrow-derived mesenchymal stem cells under oxidative stress: combination of vitamin C and graphene foams

doi: 10.18632/oncotarget.12456

Figure Lengend Snippet: ( A ) Representative image of BM-MSCs 5 days after seeding. ( B – D ) Characterization of BM-MSCs by flow cytometry. The majority of the cells are CD105 + , CD44 + and CD29 + , which are typical characteristic phenotypes of BM-MSCs. ( E ) BM-MSC can be differentiated into adipogenic and myogenic lineages. Adipogenic differentiation was characterized by Oil Red O staining, while myogenic differentiation was evidenced by the formation of myotubes stained with crystal violet. ( F ) Representative image of immunostaining of BM-MSCs on GF scaffold, stained by anti-β-tubulin (green) and DAPI for nucleus (blue). Effects of different concentrations of VC (5 to 100 μg/ml, 5 days) ( G ) and H 2 O 2 exposure (0.1 to 2 mM, 24 hours) ( H ) on cell viability of BM-MSCs cultured for 5 days, measured by MTT assay. ( I ) Cell viability of BM-MSCs in the five experimental groups. Data were presented as mean ± SEM. * p < 0.05 vs control.

Article Snippet: Antibodies used in the study were as follows: CD105-FITC (MCA1557FT, Serotec, Oxford, UK), CD44-FITC (MCA643FA, Serotec), and CD29-FITC (MCA1949FT, Serotec).

Techniques: Flow Cytometry, Staining, Immunostaining, Cell Culture, MTT Assay, Control

Journal: Cytotechnology

Article Title: Role of nanoparticles in osteogenic differentiation of bone marrow mesenchymal stem cells

doi: 10.1007/s10616-019-00353-y

Figure Lengend Snippet: Flow cytometry analysis of BM-MSCs a cluster of differentiation (CD) 73 (89.8%), b CD105 (85.7%), c CD90 (83.5%), d CD34 (8.1%) and e CD45 (0.6%), antibodies; where CD73, CD105 and CD90 stand for the fluorescent-tagged antibodies used to detect the surface antigens that are positively expressed on mesenchymal stem cells (MSCs), while CD34 and CD45 stand for the fluorescent-tagged antibodies used to detect the cell surface antigens that are only expressed on the hematopoietic stem cells and are negatively expressed on MSCs. The horizontal line in each panel represents the channel in the flow cytometry that detects the fluorescent-tagged antibodies used for detection of the expression of these markers on the MSCs

Article Snippet: In brief, BM-MSCs of third passage were released from culture flasks using trypsin/EDTA digestion, suspended in PBS containing 0.5% bovine serum albumin (BSA)/2 mmol EDTA and analyzed for surface markers expression using anti-rat phycoerythrin (PE)-conjugated CD73 and anti-rat PE-conjugated CD90 antibody (R&D Systems,UK), anti-rat fluorescein isothiocyanate (FITC)-labeled CD105 antibody (MiltenyiBiotec, Germany), anti-rat FITC-labeled CD45 antibody and anti-rat PE-conjugated CD34 antibody (Beckman Coulter Co.,USA).

Techniques: Flow Cytometry, Expressing

Journal: Laryngoscope Investigative Otolaryngology

Article Title: On the in vivo origin of human nasal mesenchymal stem cell cultures

doi: 10.1002/lio2.472

Figure Lengend Snippet: Antibodies used for immunofluorescence

Article Snippet: Mouse anti‐CD105 , Bio‐Rad , Cat# MCA1557T; AB_1100559 , 1:50.

Techniques: